منابع مشابه
[Performance evaluation of affinity column mediated immunometric assay for tacrolimus].
BACKGROUND Therapeutic drug monitoring (TDM) of tacrolimus is essential because of narrow therapeutic range and poor correlation of dose to blood concentration. Affinity Column Mediated Immunometric Assay (ACMIA) does not require a pretreatment steps in measurement of tacrolimus. In this study, we evaluated the performance of tacrolimus assay using ACMIA (Dimension RxL Max, Dade Behring). MET...
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Aggregation is a common mechanism of compound-mediated assay interference encountered in high-throughput screening (HTS) and follow-up experiments. Compounds that form aggregates in situ can nonspecifically perturb biomolecules in biochemical and cell-based assays. Nonspecific bioactivity from aggregation can waste significant resources when unaccounted for in assay design and readout interpret...
متن کاملA highly sensitive affinity-column-mediated immunometric assay, as exemplified by digoxin.
We describe a highly sensitive heterogeneous enzyme-linked immunoassay in which digoxin is used as the model analyte. An excess of enzyme-labeled monovalent antibody is incubated with sample containing the analyte such that all analyte is rapidly and quantitatively bound. Excess antibody that does not acquire a antigen in its binding site is rapidly removed from the mixture by passage through a...
متن کاملEvaluation of nicked human chorionic gonadotropin content in clinical specimens by a specific immunometric assay.
We report the development and characterization of an IRMA for the direct measurement of nicked human chorionic gonadotropin (hCGn) in blood and urine. hCGn derived from a reference preparation of hCG used as an immunogen elicits monoclonal antibodies (mAbs) with enhanced recognition of human luteinizing hormone epitopes. The most specific assay for pregnancy hCGn is an IRMA composed of one mAb ...
متن کاملRapid, automated quantification of total human chorionic gonadotropin in serum by a chemiluminescent enzyme immunometric assay.
Total human chorionic gonadotropin (hCG-hCG beta) in serum was assayed with the immulite system, a fully automated random-access immunoassay analyzer that has a unique centrifugal procedure for solid-phase washing and a chemiluminescent detection system. The broad range of the hCG calibration curve (up to 5000 IU/L) is achieved by using a small serum sample size (5 microL), which provides suffi...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2002
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/48.4.613